partial purification of a potent immunosuppressive factor excreted from leishmania major promastigote and amastigote
Authors
abstract
recent scientific evidence indicates that distinct patterns of susceptibility in balb/c mice to leishmania major infection are attributable to the differential expansion of distinct cd4+ t-cell subsets and their cytokines production. production of the th1 cytokine ifn-g is associated with resistance, whereas production of the th2 cytokines il-4 and il-10 are associated with extreme susceptibility. the major host immune defense mechanism against leishmania is activation of macrophages by inf-γ derived from t cells. the inability of susceptible hosts to mount the immune response necessary to activate macrophage and destroy the parasites may be due to the parasite-specific proteins that are able to suppress the immune system. in the present study, we have semi-purified the excreted antigens of leishmania major promastigote and amastigote by column chromatography. the isolated fraction showed a potent immunosuppressive activity on normal balb/c mice lymphocytes stimulated with mitogens. fifteen microgram of the isolated fraction caused 81% suppression of lymphocyte proliferation. these data may suggest that the parasite by secreting immunosuppressive factor down regulate the immune system and as a result survive in the body
similar resources
Partial Purification of a Potent Immunosuppressive Factor Excreted from Leishmania major Promastigote and Amastigote
Recent scientific evidence indicates that distinct patterns of susceptibility in BALB/c mice to Leishmania major infection are attributable to the differential expansion of distinct CD4+ T-cell subsets and their cytokines production. Production of the Th1 cytokine IFN-g is associated with resistance, whereas production of the Th2 cytokines IL-4 and IL-10 are associated with extreme susceptibili...
full textThe structure of Leishmania major amastigote lipophosphoglycan.
Intracellular amastigotes of Leishmania major produce 6 x 10(4) copies/cell of a lipophosphoglycan (LPG) that is structurally distinct from the LPG produced by the extracellular promastigote form of L. major, Leishmania donovani, and Leishmania mexicana (reviewed by McConville, M. J. (1991) Cell Biol. Int. Rep. 15, 779-798). L. major amastigote LPG is composed of a lysoalkyl phosphatidylinosito...
full textIdentification of Proteins in Promastigote and Amastigote-like Leishmania Using an Immunoproteomic Approach
BACKGROUND The present study aims to identify antigens in protein extracts of promastigote and amastigote-like Leishmania (Leishmania) chagasi syn. L. (L.) infantum recognized by antibodies present in the sera of dogs with asymptomatic and symptomatic visceral leishmaniasis (VL). METHODOLOGY/PRINCIPAL FINDINGS Proteins recognized by sera samples were separated by two-dimensional electrophores...
full textAntiproliferative, Ultrastructural, and Physiological Effects of Amiodarone on Promastigote and Amastigote Forms of Leishmania amazonensis
Amiodarone (AMIO), the most frequently antiarrhythmic drug used for the symptomatic treatment of chronic Chagas' disease patients with cardiac compromise, has recently been shown to have also specific activity against fungi, Trypanosoma cruzi and Leishmania. In this work, we characterized the effects of AMIO on proliferation, mitochondrial physiology, and ultrastructure of Leishmania amazonensi...
full textLeishmania donovani development in Phlebotomus argentipes: comparison of promastigote- and amastigote-initiated infections
Leishmania parasites alternate in their life cycle between promastigote stages that develop in the gut of phlebotomine sand flies and amastigotes residing inside phagocytic cells of vertebrate hosts. For experimental infections of sand flies, promastigotes are frequently used as this way of infection is technically easier although ingestion of promastigotes by sand flies is unnatural. Here we a...
full textApoptotic effect of morphine, imiquimod and nalmefene on promastigote , infected and uninfected macrophages with amastigote of Leishmania major by flow cytometry
The parasites of genus Leishmania are the causative agents of one of the most widespread and devastating diseases. According to follow-up data, these medications may provoke adverse drug reactions, drug resistance, relapse as well as financial burden. The mechanism of action of opioid drugs are primarily exerted via transmembrane G-protein coupled receptors. One of the potent synthetic immunomo...
full textMy Resources
Save resource for easier access later
Journal title:
iranian biomedical journalجلد ۸، شماره ۲، صفحات ۹۵-۹۹
Hosted on Doprax cloud platform doprax.com
copyright © 2015-2023